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| SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR. |
| ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649 |
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| CAVALCANTI,MARIANA B.; FERNANDES,THIAGO S.; SILVA,EDVANE B.; AMARAL,ADEMIR. |
| The aim of this research was to evaluate the relationship between p53 protein levels and absorbed doses from in vitro irradiated human lymphocytes. For this, samples of blood from 23 donors were irradiated with 0.5; 1; 2; and 4 Gy from a Cobalt-60 source, and the percentages of lymphocytes expressing p53 were scored using Flow Cytometry. The subjects were divided into 3 groups, in accordance with the p53 levels expressed per radiation dose: low (Group I), high (Group II), and excessive levels (Group III). For all groups, the analyses showed that the p53 expression levels increase with the absorbed dose. Particularly for groups I and II, the correlation between this protein expression and the dose follows the linear-quadratic model, such as for radioinduced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological Dosimetry; Cellular radiobiology; Flow Cytometry; P53 protein; Radiosensitivity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401783 |
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